Plasmid_Backbone

Part:BBa_K228867:Design

Designed by: Lin Min   Group: iGEM09_PKU_Beijing   (2009-09-22)


pSB1A2-lacZ screening plasmid


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2345
    Illegal NheI site found at 14
    Illegal NheI site found at 37
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
    Illegal NotI site found at 2351
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 2345
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found at 2345
    Plasmid lacks a suffix.
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found at 2345
    Plasmid lacks a suffix.
    Illegal XbaI site found at 2360
    Illegal SpeI site found at 2
    Illegal PstI site found at 303
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1384


Design Notes

when cut this part for insert and ligate it into a vetor, the insert and vector may have the similar sizes which may disturb the ligation.


Source

part registery 2009 distribution & PKU 2009 igem team

References